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Twist Bioscience pet28a expression vectors
Pet28a Expression Vectors, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a+expression+vectors/pet28a/pm40553903-59-13-10
Average 90 stars, based on 1 article reviews
pet28a expression vectors - by Bioz Stars, 2026-09
90/100 stars

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Article Title: The product specificities of terpinolene synthase, from cannabis sativa, reveals the plasticity of the terpene synthase active site.
Article Snippet: Cannabis sativa is a high-value plant renowned for its diverse chemical composition and abundant terpene content, contributing to its unique aroma, flavour, and therapeutic effects.. Terpenes significantly influence consumer preference for C. sativa products, driving scientific interest in optimising terpene expression profiles and shaping the selective breeding of terpene profiles in C. sativa cultivars.. In particular, the monoterpene, terpinolene, is influential in defining the sensory and therapeutic qualities of many C. sativa strains due to its woody, citrus-like aroma.

Expressing:

Article Title: The product specificities of terpinolene synthase, from cannabis sativa, reveals the plasticity of the terpene synthase active site.
Article Snippet: Cannabis sativa is a high-value plant renowned for its diverse chemical composition and abundant terpene content, contributing to its unique aroma, flavour, and therapeutic effects.. Terpenes significantly influence consumer preference for C. sativa products, driving scientific interest in optimising terpene expression profiles and shaping the selective breeding of terpene profiles in C. sativa cultivars.. In particular, the monoterpene, terpinolene, is influential in defining the sensory and therapeutic qualities of many C. sativa strains due to its woody, citrus-like aroma.

Article Title: Molecular Basis for Peptide Nitration by a Novel Cytochrome P450 Enzyme in RiPP Biosynthesis
Article Snippet: Expression and purification of redox proteins The PdX (#85083) and PdR (#85084) plasmids were obtained from Addgene. .. The genes for CPR, SpFdX, and SpFdR were cloned into pET28a expression vectors (Twist Bioscience) using methods based on previously reported protocols.1-4 Expression and purification procedures were adapted from established protocols.1-5 All plasmids were transformed into E. coli BL21 (DE3) cells, which were cultured in Luria-Bertani medium supplemented with kanamycin (50 μg/mL) at 37 °C until OD600 reached 0.6. ..

Sequencing:

Article Title: The product specificities of terpinolene synthase, from cannabis sativa, reveals the plasticity of the terpene synthase active site.
Article Snippet: Cannabis sativa is a high-value plant renowned for its diverse chemical composition and abundant terpene content, contributing to its unique aroma, flavour, and therapeutic effects.. Terpenes significantly influence consumer preference for C. sativa products, driving scientific interest in optimising terpene expression profiles and shaping the selective breeding of terpene profiles in C. sativa cultivars.. In particular, the monoterpene, terpinolene, is influential in defining the sensory and therapeutic qualities of many C. sativa strains due to its woody, citrus-like aroma.

Clone Assay:

Article Title: Molecular Basis for Peptide Nitration by a Novel Cytochrome P450 Enzyme in RiPP Biosynthesis
Article Snippet: Expression and purification of redox proteins The PdX (#85083) and PdR (#85084) plasmids were obtained from Addgene. .. The genes for CPR, SpFdX, and SpFdR were cloned into pET28a expression vectors (Twist Bioscience) using methods based on previously reported protocols.1-4 Expression and purification procedures were adapted from established protocols.1-5 All plasmids were transformed into E. coli BL21 (DE3) cells, which were cultured in Luria-Bertani medium supplemented with kanamycin (50 μg/mL) at 37 °C until OD600 reached 0.6. ..

Purification:

Article Title: Molecular Basis for Peptide Nitration by a Novel Cytochrome P450 Enzyme in RiPP Biosynthesis
Article Snippet: Expression and purification of redox proteins The PdX (#85083) and PdR (#85084) plasmids were obtained from Addgene. .. The genes for CPR, SpFdX, and SpFdR were cloned into pET28a expression vectors (Twist Bioscience) using methods based on previously reported protocols.1-4 Expression and purification procedures were adapted from established protocols.1-5 All plasmids were transformed into E. coli BL21 (DE3) cells, which were cultured in Luria-Bertani medium supplemented with kanamycin (50 μg/mL) at 37 °C until OD600 reached 0.6. ..

Transformation Assay:

Article Title: Molecular Basis for Peptide Nitration by a Novel Cytochrome P450 Enzyme in RiPP Biosynthesis
Article Snippet: Expression and purification of redox proteins The PdX (#85083) and PdR (#85084) plasmids were obtained from Addgene. .. The genes for CPR, SpFdX, and SpFdR were cloned into pET28a expression vectors (Twist Bioscience) using methods based on previously reported protocols.1-4 Expression and purification procedures were adapted from established protocols.1-5 All plasmids were transformed into E. coli BL21 (DE3) cells, which were cultured in Luria-Bertani medium supplemented with kanamycin (50 μg/mL) at 37 °C until OD600 reached 0.6. ..

Cell Culture:

Article Title: Molecular Basis for Peptide Nitration by a Novel Cytochrome P450 Enzyme in RiPP Biosynthesis
Article Snippet: Expression and purification of redox proteins The PdX (#85083) and PdR (#85084) plasmids were obtained from Addgene. .. The genes for CPR, SpFdX, and SpFdR were cloned into pET28a expression vectors (Twist Bioscience) using methods based on previously reported protocols.1-4 Expression and purification procedures were adapted from established protocols.1-5 All plasmids were transformed into E. coli BL21 (DE3) cells, which were cultured in Luria-Bertani medium supplemented with kanamycin (50 μg/mL) at 37 °C until OD600 reached 0.6. ..



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